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- Title
Characterization of CD4+ Memory T Cell Responses Directed against Common Respiratory Pathogens in Peripheral Blood and Lung.
- Authors
De Bree, Godelieve J.; Daniels, Hans; Van Schilfgaarde, Muriel; Jansen, Henk M.; Out, Theo A.; Van Lier, René A. W.; Jonkers, René E.
- Abstract
Background. We investigated CD4+ memory T cell responses to influenza virus (FLU), respiratory syncytial virus (RSV), and nontypeable Haemophilus influenzae (NTHi). Methods. The precursor frequencies of antigen-specific CD4+ cells were determined by in vitro expansion of peripheral blood mononuclear cells from healthy individuals (np9) and patients with chronic obstructive pulmonary disease (COPD; np16). The expression of CD27 and CCR7 and the production of interferon (IFN)-g and interleukin-2 was measured directly ex vivo. Furthermore, the phenotypic and functional properties of CD4+ T cells residing in the lung were analyzed and compared with those of circulating CD4+memory cells from the same donors ( np8). Results. FLU-, RSV-, and NTHi-specific CD4+ memory T cells circulated at low frequencies in the peripheral blood of healthy individuals and patients. RSV- and NTHi-specific CD4+ T cells had a memory phenotype with moderate to high CD27 and CCR7 expression. In contrast to the low frequencies of circulating FLU-specific CD4+ T cells, we found an enrichment of differentiated CD4+ FLU-specific cells and high IFN-γ expression in CD4+ memory cells in lung tissue. Conclusion. No gross defects were found in circulating CD4+ memory cells specific for pathogens associated with COPD. However, the large differentiated CD4+ memory T cell pool residing in the lung may contribute to a large extent to local antiviral immunological protection.
- Subjects
RESPIRATORY infections; RESPIRATORY diseases; T cells; PATHOGENIC microorganisms; BLOOD; INFLUENZA viruses; COMMUNICABLE diseases
- Publication
Journal of Infectious Diseases, 2007, Vol 195, Issue 11, p1718
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/517612