We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Optimized Adaptor Polymerase Chain Reaction Method for Efficient Genomic Walking.
- Authors
Peng XU; Rui-Ying HU; Xiao-Yan DING
- Abstract
Genomic walking is one of the most useful approaches in genome-related research. Three kinds of PCR-based methods are available for this purpose. However, none of them has been generally applied because they are either insensitive or inefficient. Here we present an efficient PCR protocol, an optimized adaptor PCR method for genomic walking. Using a combination of a touchdown PCR program and a special adaptor, the optimized adaptor PCR protocol achieves high sensitivity with low background noise. By applying this protocol, the insertion sites of a gene trap mouse line and two gene promoters from the incompletely sequenced Xenopus laevis genome were successfully identified with high efficiency. The general application of this protocol in genomic walking was promising. Edited by Ming-Hua XU
- Subjects
POLYMERASE chain reaction; GENETICS; GENOMES; PROMOTERS (Genetics); CYTOLOGY; RESEARCH
- Publication
Acta Biochimica et Biophysica Sinica, 2006, Vol 38, Issue 8, p571
- ISSN
1672-9145
- Publication type
Article
- DOI
10.1111/j.1745-7270.2006.00194.x