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- Title
The correlation between sPD-1/sPD-L1 and T cells in patients with HBV-related acute-on -chronic liver failure.
- Authors
GULISHATI, Haimiti; ZHU Yuejie; XIE Xinru; GUO Wenhong; DING Jianbing; LU Xiaobo; ZHANG Fengbo
- Abstract
Objective: To investigate the expression levels of soluble programmed cell death protein1 (sPD-1) and soluble programmed cell death ligandl (sPD-L1), T cells, interleukin-10 (IL-10) and transforming growth factor-β (TGF-β) in peripheral blood of patients with hepatitis B virus (HBV) -related acute-on-chronic liver failure (ACLF) and the correlation between each index. Methods: According to the inclusion and exclusion criteria, the data and peripheral blood samples of 71 patients diagnosed with HBV-ACLF were collected. According to the 28-day follow-up, the subjects were divided into survival group (40 cases) and death group (31 cases). The proportion of CD4+T and CD8 + T cells in peripheral blood was detected by flow cytometry. The expression levels of sPD-1 and sPD-Ll in serum were detected by cytometric bead array (CBA). Enzyme-linked immunosorbent assay was used to detect the expression of TGF-β and IL-10 in the serum of the subjects. Pearson method was used to analyze the correlation between serum sPD-1 and sPD-Ll and MELD score, T lymphocyte level, and cytokine level in patients with HBV- ACLF. Results: The percentages of CD4+T cells, sPD-1, sPD-Ll, IL-10 and TGF-β in peripheral blood and serum of the death group were higher than those of the survival group, while the percentage of CD8+T cells was lower than that of the survival group (all P<0.05). Correlation analysis showed that the level of sPD-1 was positively correlated with the percentages of IL-10, TGF-p and CD4+T cells, and negatively correlated with the percentage of CD8 + T cells. The level of sPD-L1 was positively correlated with model for end-stage liver disease score, IL-10, TGF-β and CD4+T, and negatively correlated with the proportion of CD8+T cells (all P<0.05).Conclusion: sPD-1 and sPD-L1 may be involved in the immune response during the occurrence and development of HBV-ACLF. By comprehensively considering the levels of sPD-1 and sPD-L1, it can provide certain clinical value for timely and effective treatment of HBV-ACLF patients.
- Subjects
T cells; LIVER failure; APOPTOSIS; PROGRAMMED death-ligand 1; ENZYME-linked immunosorbent assay; CHRONIC hepatitis B
- Publication
Journal of Hainan Medical University, 2024, Vol 30, Issue 11, p817
- ISSN
1007-1237
- Publication type
Article
- DOI
10.13210/j.cnki.jhmu.20240306.001