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- Title
Hpa1 secretion via type III secretion system in Xanthomonas oryzae pv. oryzae.
- Authors
Furutani, Ayako; Tsuge, Seiji; Oku, Takashi; Tsuno, Kazunori; Inoue, Yasuhiro; Ochiai, Hirokazu; Kaku, Hisatoshi; Kubo, Yasuyuki
- Abstract
In many Gram-negative plant pathogenic bacteria the type III secretion system (TTSS), encoded by hrp genes, is essential for pathogenicity in the host and induction of a hypersensitive reaction (HR) in nonhost plants. The expression of hrp genes has been suggested to be repressed in complex media, whereas it is induced in planta and under certain in vitro conditions. We recently reported that XOM2 medium allows efficient hrp expression by Xanthomonas oryzae pv. oryzae. In this study, we investigated hrp-dependent secretion of proteins by the bacteria in vitro. Using modified XOM2, in which bovine serum albumin was added and the pH was lowered to 6.0, we detected at least 10 secreted proteins and identified one as Hpa1. This is the first evidence of protein secretion via TTSS in X. oryzae pv. oryzae.
- Subjects
XANTHOMONAS; GRAM-negative bacteria; PATHOGENIC bacteria; SECRETION; PLANT diseases
- Publication
Journal of General Plant Pathology, 2003, Vol 69, Issue 4, p271
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-003-0042-2