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- Title
Expression pattern of melanin biosynthesis enzymes during infectious morphogenesis of Colletotrichum lagenarium.
- Authors
Tsuji, Gento; Tsuge, Seiji; Shiraishi, Tomonori; Kubo, Yasuyuki
- Abstract
A plant pathogenic fungus, Colletotrichum lagenarium, produces melanin in the appressorium, a highly specialized infection structure. Appressorium melanization is essential for penetrating host leaves. This fungus shows synchronous cellular differentiation from conidia, and two types of cellular differentiation can be controlled by the incubation temperature. When incubated at 24°C, conidia germinate and produce darkly melanized appressoria. In contrast, at 32°C conidia germinate and elongate germ tubes but do not produce appressoria. In the infection-related morphogenesis of this fungus, melanin is confined to appressoria and is observed in neither conidia nor germ tubes. Our previous RNA gel blot analysis revealed that melanin biosynthesis structural genes were expressed during the early stage of conidial germination regardless of appressorium formation, suggesting the involvement of posttranscriptional regulation for appressorium melanization. In this study, we examined temporal translational patterns of melanin biosynthetic enzymes during the development of germinating conidia incubated at 24° and 32°C. Western blot analysis revealed that two melanin biosynthesis enzymes, scytalone dehydratase (Scd1p) and 1,3,8-trihydroxynaphthalene reductase (Thr1p), were expressed in appressorium-differentiating conidia incubated at 24°C but not in appressorium-nondifferentiating conidia at 32°C.
- Subjects
MELANINS; BIOSYNTHESIS; ENZYMES; MORPHOGENESIS; COLLETOTRICHUM lagenarium
- Publication
Journal of General Plant Pathology, 2003, Vol 69, Issue 3, p169
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-002-0032-9