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- Title
Neural Differentiation of Mouse Neural Stem Cells as a Tool to Assess Developmental Neurotoxicity of Drinking Water in Taihu Lake.
- Authors
Zhou, Yang; Fu, Yu; Bai, Zhendong; Li, Peixin; Zhao, Bo; Han, Yuehua; Xu, Ting; Zhang, Ningyan; Lin, Lin; Cheng, Jian; Zhang, Jun; Zhang, Jing
- Abstract
In this study, we used neural stem cells (NSCs) as a toxicology tool to assess the potential developmental neurotoxicity of drinking water from Taihu Lake. We found that the condensed drinking water could inhibit the proliferation and differentiation of NSCs, especially the tap water. Inductively coupled plasma mass spectrometry and high-performance liquid chromatography analysis showed that nickel was detected in the tap water with a high concentration. Our study revealed that nickel could inhibit NSCs proliferation and differentiation, which is induced not only by the intracellular reactive oxygen species generation, but also by the protein levels upregulation of p-c-Raf, p-MEK1/2 and p-Erk1/2 through the axon guidance signal pathways. These findings will provide a new way of research insight for investigation of nickel-induced neurotoxicity. Meanwhile, our test method confirmed the feasibility and reliability of stem cell assays for developmental neurotoxicity testing.
- Subjects
NEURAL stem cells; DRINKING water; INDUCTIVELY coupled plasma mass spectrometry; NEUROTOXICOLOGY
- Publication
Biological Trace Element Research, 2019, Vol 190, Issue 1, p172
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-018-1533-5