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- Title
A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators.
- Authors
Cardoso, T. C.; Sakamoto, S. S.; Stockmann, D.; Souza, T. F. B.; Ferreira, H. L.; Gameiro, R.; Vieira, F. V.; Louzada, M. J. Q.; Andrade, A. L.; Flores, E. F.
- Abstract
In this study, derived complex carcinoma ( CC) and simple carcinoma ( SC) cell lines were established and cultured under two-dimensional ( 2D) and three-dimensional ( 3D) conditions. The 3D was performed in six-well AlgiMatrix™ (LifeTechnologies®, Carlsbad, CA, USA) scaffolds, resulting in spheroids sized 50-125 µm for CC and 175-200 µm for SC. Cell viability was demonstrated up to 14 days for both models. Epidermal growth factor receptor ( EGFR) was expressed in CC and SC in both systems. However, higher mRNA and protein levels were observed in SC 2D and 3D systems when compared with CC ( P < 0.005). The connective tissue modulators, metalloproteinases-1, -2, -9 and -13 (MMPs), relaxin receptors 1 and 2 ( RXR1 and RXR2) and E-cadherin ( CDH1) were quantitated. All were upregulated similarly when canine mammary tumour (CMT)-derived cell lines were cultured under 3D AlgiMatrix, except CDH1 that was downregulated ( P < 0.005). These results are promising towards the used of 3D system to increase a high throughput in vitro canine tumour model.
- Subjects
CARCINOMA; CELL lines; EPIDERMAL growth factor receptors; METALLOPROTEINASES; CADHERINS
- Publication
Veterinary & Comparative Oncology, 2017, Vol 15, Issue 2, p582
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/vco.12202