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- Title
Molecular mapping of the Aegilops speltoides-derived leaf rust resistance gene Lr36 in common wheat (Triticum aestivum).
- Authors
Pourkhorshid, Zahra; Dadkhodaie, Ali; Niazi, Ali
- Abstract
Leaf rust caused by Puccinia triticina Eriks. is the most prevalent wheat rust worldwide and occurs annually wherever wheat is grown. The most economical and environmentally friendly method to control this fungal disease is genetic resistance, which is achieved through deploying effective resistance genes. Tightly-linked molecular markers facilitate gene tagging and their deployment with other resistance genes, which in turn contribute to durable leaf rust resistance. The leaf rust resistance gene Lr36 derived from Aegilops speltoides Taush and introgressed into hexaploid wheat by backcrossing, is located on chromosome 6BS. Despite detection of low frequencies of virulence for this gene, no tightly-linked marker is available in disease resistance breeding. Therefore, this research aimed at analysis of simple sequence repeats (SSR) markers linked with Lr36 in 171 individuals of an F2 population from a cross between the Lr36-carrying line (ER84018) and the susceptible cultivar; Boolani. Of 36 primer pairs on chromosome 6BS tested for polymorphism in parents and resistant and susceptible bulks, eight were polymorphic of which the markers Xgwm88 and Xcfd13 flanked Lr36 by 3.8 and 5.2 cM, respectively. The identified markers were validated on 35 genotypes with different genetic backgrounds including few Australian wheat leaf rust differential sets, Iranian landraces and cultivars, and several cultivars and/or breeding lines from Switzerland and Afghanistan, which confirmed that they can be used in selection for Lr36 in wheat breeding programs.
- Subjects
AFGHANISTAN; SWITZERLAND; GENE mapping; WHEAT; MICROSATELLITE repeats; AEGILOPS; WHEAT breeding; WHEAT rusts; RUST diseases
- Publication
Euphytica, 2022, Vol 218, Issue 3, p1
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-022-02975-4