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- Title
Molecular and cytological characterization of a cytoplasmic-specific mutant in pima cotton (Gossypium barbadenseL.).
- Authors
Karaca, Mehmet; Saha, Sukumar; Callahan, Franklin E.; Jenkins, Johnie N.; Read, John J.; Percy, Richard G.
- Abstract
A cytoplasmic mutant ofGossypium barbadenseL.,cyt-Vwas characterized at the morphological, cellular, genetic and molecular levels using comparison analysis withv7 v7, a nuclear virescent mutant to identify molecular effects of thecyt-Vmutation. The yellow phenotype was specific only to leaves in thecyt-Vmutant (CM-1-90) but the same phenotype was present in both leaves and cotyledons ofv7 v7, a nuclear virescent mutant, suggesting thatcyt-Vandv7 v7, had different organ-specific gene actions. Chlorophyll and carotenoid levels of CM-1-90, CM-1-90 × PS-7 and CM-1-90 ×v7 v7 true leaves were significantly lower than in the true leaves of PS-7 × CM-1-90,v7 v7 × CM-1-90 and PS-7. Anatomical studies of chloroplast showed that CM-1-90, CM-1-90 × PS-7 and CM-1-90 ×v7 v7 lacked grana in the thylakoids of the mesophyll cells. This indicated that chlorophyll and carotenoid levels correlated with chloroplast structure. SDS-PAGE analysis of thylakoid preparations revealed decreases of several granalocalized PSII proteins in CM-1-90, CM-1-90 × PS-7 and CM-1-90 ×v7 v7. cDNA-AFLP differential display studies identified several differentially expressed transcripts in the leaves of reciprocal crosses (PS-7 × CM-1-90,v7 v7 × CM-1-90 and CM-1-90 × PS-7 and CM-1-90 ×v7 v7), including one possessing a high sequence homology to apsbAgene. Western blot analysis further confirmed the absence of D1 protein encoded bypsbAin CM-1-90 × PS-7' CM-1-90 ×v7 v7 and CM-1-90 true leaves. Overall, we studiedcyt-Vandv7 v7 that both are developmental mutants, as all the virescents of cotton mutants, and as such it was difficult to separate cause and effect in the observation; however, we verified that the source ofcyt-Vmutation was in chloroplast and elucidated that its gene action was different fromv7 v7. Results indicated thatcyt-Vis inherited as a single gene but it affects several chloroplast and nucleus-encoded genes. We identified several transcripts that associated with thecyt-Vmutation. This study also suggested that chloroplast-encoded gene products might affect the expression of nuclear genes, possibly at the transcriptional stage.
- Subjects
SEA Island cotton; DNA; CHLOROPLASTS; PLANT mutation; PLANT genetics; AGRICULTURE
- Publication
Euphytica, 2004, Vol 139, Issue 3, p187
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-004-3032-3