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- Title
Genetic Basis Identification of a NLR Gene, TaRGA5-like , That Confers Partial Powdery Mildew Resistance in Wheat SJ106.
- Authors
Liu, Xiaoying; Yang, Chenxiao; Wu, Siqi; Dong, Huixuan; Wang, Guangyu; Han, Xinyue; Fan, Baoli; Shang, Yuntao; Dang, Chen; Xie, Chaojie; Wang, Zhenying
- Abstract
Wheat powdery mildew is an important fungal disease that seriously jeopardizes wheat production, which poses a serious threat to food safety. SJ106 is a high-quality, disease-resistant spring wheat variety; this disease resistance is derived from Wheat-wheatgrass 33. In this study, the powdery mildew resistance genes in SJ106 were located at the end of chromosome 6DS, a new disease resistance locus tentatively named PmSJ106 locus. This interval was composed of a nucleotide-binding leucine-rich repeat (NLR) gene cluster containing 19 NLR genes. Five NLRs were tandem duplicated genes, and one of them (a coiled coil domain–nucleotide binding site–leucine-rich repeat (CC-NBS-LRR; CNL) type gene, TaRGA5-like) expressed 69–836-fold in SJ106 compared with the susceptible control. The genome DNA and cDNA sequences of TaRGA5-like were amplified from SJ106, which contain several nucleotide polymorphisms in LRR regions compared with susceptible individuals and Chinese Spring. Overexpression of TaRGA5-like significantly increased resistance to powdery mildew in susceptible receptor wheat Jinqiang5. However, Virus induced gene silence (VIGS) of TaRGA5-like resulted in only a small decrease of SJ106 in disease resistance, presumably compensated by other NLR duplicated genes. The results suggested that TaRGA5-like confers partial powdery mildew resistance in SJ106. As a member of the PmSJ106 locus, TaRGA5-like functioned together with other NLR duplicated genes to improve wheat resistance to powdery mildew. Wheat variety SJ106 would become a novel and potentially valuable germplasm for powdery mildew resistance.
- Subjects
POWDERY mildew diseases; WHEAT; GENES; NATURAL immunity; MYCOSES; GENE silencing
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 12, p6603
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25126603