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- Title
Detection and classification of allexiviruses from garlic in China.
- Authors
J. Chen; H.-Y. Zheng; J. F. Antoniw; M. J. Adams; J.-P. Chen; L. Lin
- Abstract
Summary. Degenerate primers for RT-PCR were designed and used to amplify genome fragments ( c. 750?nt in the coat protein-ORF6 region) of allexiviruses from a total of 28 garlic samples from 24 provinces in China. Many samples contained more than one distinct sequence. A total of 60 different sequences were obtained. Phylogenetic analysis and two-way comparisons were used to assess the status of the sequences and to re-examine the criteria for distinguishing species within the genus. Most of the sequences could be allocated to either Garlic virus D or Garlic virus X on the basis of sequence similarity but some appeared to be intermediate between existing species. There were no sequences of Garlic virus C or Shallot virus X. A comparison with the related genera Carlavirus, Foveavirus and Potexvirus suggests that the published allexivirus species demarcation criteria may have been drawn too tightly and should be re-examined.
- Subjects
CHINA; GARLIC; VIRUS diseases; GENOMES
- Publication
Archives of Virology, 2004, Vol 149, Issue 3, p435
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-003-0234-2