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- Title
Genetic diversity of apple fruit crinkle viroid populations in Japanese persimmons and the infectivity of a predominant sequence variant to tomato plants.
- Authors
Hataya, Tatsuji
- Abstract
Apple fruit crinkle viroid (AFCVd) infects apple, hop, and oriental persimmon plants as natural hosts. The genetic diversity of AFCVd populations in persimmons is less well understood than that in apples and hops. In this study, AFCVd was detected in the pericarp of persimmon fruits harvested from a private garden tree in Hyogo, Osaka, Kyoto, and Shiga Prefectures of the Kinki region, Japan. Sequence analyses revealed that the AFCVd population in each persimmon sample comprised at least 2–5 sequence variants. The infectivity of one or two sequence variants in each persimmon sample was examined using dimeric linear RNA transcribed from a cDNA clone containing the dimer of each consensus sequence. Three predominant variants (perHS1, perOK1, and perKU1) had infectivity in tomato plants; however, one variant (perSN1) and a rare variant (perHS2) did not. In contrast, another variant (perSN2) in the persimmon from Shiga was confirmed to be infectious because it had a sequence identical to the predominant infectious variant, perOK1. Examination of the AFCVd population in the Shiga persimmon using restriction enzyme digestion and direct sequencing indicated that the predominant variant was perSN2 rather than perSN1. The results show that the AFCVd populations in persimmons are genetically diverse, with at least one predominant sequence variant identified in each persimmon being capable of infecting tomatoes, and a rare variant that may be incapable of infecting tomatoes.
- Subjects
GENETIC variation; PERSIMMON; FRUIT; HOST plants; ORNAMENTAL trees; APPLES; TOMATOES
- Publication
European Journal of Plant Pathology, 2024, Vol 169, Issue 2, p273
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-024-02825-0