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- Title
Improved quantitative method for isolating Calonectria ilicicola from naturally infested soils.
- Authors
Ochi, Sunao; Nakagawa, Akio
- Abstract
We developed an improved procedure for quantitative isolation of Calonectria ilicicola from naturally infested soils. The selective medium contained 20 g l-sorbose, 4 g yeast extract, 12.5 mg flutolanil, 1.5 mg thiabendazole, 40 mg chlortetracycline hydrochloride, 10 mg chloramphenicol, 1 mL tergitol, 20 g agar, and up to 1 L with distilled water. Soil samples are wet-sieved (mesh size 0.250-0.038 mm) and then surface-sterilized for 30 s with 0.25 % NaClO solution before incubation with this medium. Compared with previously reported methods, this method was more effective for isolating C. ilicicola, and the amount of contamination was decreased.
- Subjects
SOYBEAN; YEAST; PLANT diseases; SOILS; CARBON; PLANT growth
- Publication
Journal of General Plant Pathology, 2012, Vol 78, Issue 3, p147
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-012-0377-7