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- Title
A simple method for long-term cryopreservation of Calonectria ilicicola on barley grains.
- Authors
Ochi, Sunao; Nakagawa, Akio
- Abstract
We developed a simple method for long-term preservation of the soybean red crown rot fungus, Calonectria ilicicola, using barley grains. Autoclaved barley grains were inoculated with the fungus, then incubated at 25°C for 1 month. After incubation, grains were dried to approximately 3% moisture content, and stored at 4, −20, or −80°C for 3 years. C. ilicicola preserved on barley grains at −80°C remained viable without any change in mycelial growth and virulence. These results showed that C. ilicicola can be successfully cryopreserved for extended periods on barley grains at −80°C. We also confirmed that cultures preserved on barley grains are suitable for direct use without further manipulation as inocula in pathogenicity tests.
- Subjects
CRYOPRESERVATION of organs, tissues, etc.; CRYOPRESERVATION of cells; BARLEY; SOYBEAN diseases &; pests; CALONECTRIS
- Publication
Journal of General Plant Pathology, 2010, Vol 76, Issue 2, p112
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-009-0217-6