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- Title
Nucleoside und Nucleotide. Teil 14. Synthese des Octanucleosidheptaphosphats d(G-A-T-T-T-T-G-A).
- Authors
Bühlmayer, Peter; Graf, Georg; Waldmeier, Felix; Tamm, Christoph
- Abstract
Nucleosides and Nucleotides. Part 14. Synthesis of the Octanucleosideheptaphosphate d(G-A-T-T-T-T-G-A) The synthesis of the oligonucleotide d(G-A-T-T-T-T-G-A) by the diester method is described. The condensations were performed using dinucleotide blocks carrying suitable protecting groups. The intermediary products were d(G-A), d(G-A-T-T) and d(G-A-T-T-T-T). These intermediates and the final product were characterized by UV. spectroscopy, high pressure liquid chromatography (HPLC.) and enzymatic degradation. The cleavage products were separated and analyzed by paper chromatography or HPLC. with an anion exchange column. They yielded the corresponding nucleosides and nucleotides in the correct ratios.
- Publication
Helvetica Chimica Acta, 1980, Vol 63, Issue 8, p2469
- ISSN
0018-019X
- Publication type
Article
- DOI
10.1002/hlca.19800630841