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- Title
Distribution and functional analysis of the two types of 8-vinyl reductase involved in chlorophyll biosynthesis in marine cyanobacteria.
- Authors
Suehiro, Haruka; Tanaka, Ryouichi; Ito, Hisashi
- Abstract
In the chlorophyll biosynthesis pathway, the 8-vinyl group of the chlorophyll precursor is reduced to an ethyl group by 8-vinyl reductase. Two isozymes of 8-vinyl reductase have been described in oxygenic photosynthetic organisms: one encoded by BciA and another by BciB. Only BciB contains an [Fe-S] cluster and most cyanobacteria harbor this form; whereas a few contain BciA. Given this disparity in distribution, cyanobacterial BciA has remained largely overlooked, which has limited understanding of chlorophyll biosynthesis in these microorganisms. Here, we reveal that cyanobacterial BciA encodes a functional 8-vinyl reductase, as evidenced by measuring the in vitro activity of recombinant Synechococcus and Acaryochloris BciA. Genomic comparison revealed that BciB had been replaced by BciA during evolution of the marine cyanobacterium Synechococcus, and coincided with replacement of Fe-superoxide dismutase (SOD) with Ni-SOD. These findings imply that the acquisition of BciA confers an adaptive advantage to cyanobacteria living in low-iron oceanic environments.
- Subjects
THEORY of distributions (Functional analysis); CHLOROPHYLL; BIOSYNTHESIS; CYANOBACTERIA; SYNECHOCOCCUS; ETHYL group
- Publication
Archives of Microbiology, 2021, Vol 203, Issue 6, p3565
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-021-02348-w