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- Title
金针菇三个锌簇转录因子鉴定与表达分析.
- Authors
黄千慧; 魏仲阳; 刘泰娇; 戴宇松; 张维瑞; 谢宝贵
- Abstract
Three zinc cluster transcription factors in Flammulina filiformis, FVZCP1, FVZCP2, and FVZCP3, were analyzed for basic properties, zinc cluster domain and phylogeny by bioinformatics tools, and then determined for their expression in mycelium, primordium, stipe, and pileus by qRT-PCR. The results showed that Fvzcp1, Fvzcp2, and Fvzcp3 distributed in different contigs, and contained 9, 14, and 10 exons, encoding 2 553 bp, 2 634 bp, and 1 743 bp, respectively. The corresponding proteins, FVZCP1, FVZCP2, and FVZCP3, were 93 763.19 Da, 98 333.89 Da, and 64 251.26 Da, respectively. Their theoretical pI are 7.10, 6.54, 6.64, respectively. They were predicted to be unstable hydrophobic proteins and localized in the nucleus. All of them had a typical zinc cluster domain, albeit in different types. In terms of expression level, Fvzcp1 was relatively higher in primordium and pileus than in mycelium; Fvzcp2 was relatively higher in primordium and stipe than in mycelium; Fvzcp3 was higher in stipe than in mycelium. Their expression levels were significantly higher in the elongation region than those in the non-elongation region.
- Subjects
IMMOBILIZED proteins; TRANSCRIPTION factors; MYCELIUM; ZINC proteins; ZINC
- Publication
Acta Edulis Fungi, 2023, Vol 30, Issue 5, p1
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.05.001