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- Title
Complete chemoenzymatic synthesis of the Forssman antigen using novel glycosyltransferases identified in Campylobacter jejuni and Pasteurella multocida.
- Authors
R Scott Houliston; Stéphane Bernatchez; Marie-France Karwaski; Robert E Mandrell; Harold C Jarrell; Warren W Wakarchuk; Michel Gilbert
- Abstract
We have identified an α1,4-galactosyltransferase (CgtD) and a β1,3-N-acetylgalactosaminyltransferase (CgtE) in the lipooligosaccharide (LOS) locus of Campylobacter jejuni LIO87. Strains that carry these genes may have the capa- bility of synthesizing mimics of the P blood group antigens of the globoseries glycolipids. We have also identified an α1,3-N-acetylgalactosaminyltransferase (Pm1138) from Pasteurella multocida Pm70, which is involved in the synthesis of an LOS-bound Forssman antigen mimic and represents the only known bacterial glycosyltransferase with this specificity. The genes encoding the three enzymes were cloned and expressed in Escherichia coli as soluble recombinant proteins that can be used to chemoenzymatically synthesize the Forssman antigen, and its biosynthetic precursors, in high yields.
- Subjects
PASTEURELLA; PASTEURELLACEAE; PASTEURELLA multocida; BRUCELLACEAE
- Publication
Glycobiology, 2009, Vol 19, Issue 2, p153
- ISSN
0959-6658
- Publication type
Article