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- Title
PCR-based assay for the detection of Curtobacterium flaccumfaciens pv.flaccumfaciens in bean seeds.
- Authors
Tegli, S.; Sereni, A.; Surico, G.
- Abstract
Aims: To develop a PCR-based protocol for the rapid, sensitive and specific detection of Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) in bean seeds. Methods and Results: A pair of PCR primers (CffFOR2-CffREV4), targeting the sequence of a cloned DNA fragment of 550 bp amplified in Repetitive-sequence-based-PCR (Rep-PCR) experiments, were designed and shown to specifically amplify a 306-bp DNA fragment using CffDNA as template. Moreover, this PCR protocol was demonstrated to successfully detect Cff in naturally infected bean seeds in 36 h. Conclusions: A specific, highly sensitive and rapid PCR assay for the detection of Cff was achieved. Significance and Impact of the Study: Cff is a seed-borne bacterium on the EPPO A2 quarantine list; this procedure may be useful for routine diagnosis of Cff, overcoming the problems of conventional techniques.
- Subjects
SEED-borne phytopathogens; DIAGNOSTIC use of polymerase chain reaction
- Publication
Letters in Applied Microbiology, 2002, Vol 35, Issue 4, p331
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1046/j.1472-765X.2002.01187.x