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- Title
Strain-level bacterial identification by CeO<sub>2</sub>-catalyzed MALDI-TOF MS fatty acid analysis and comparison to commercial protein-based methods.
- Authors
Cox, C. R.; Jensen, K. R.; Saichek, N. R.; Voorhees, K. J.
- Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid approach for clinical bacterial identification. However, current protein-based commercial bacterial ID methods fall short when differentiating closely related species/strains. To address this shortcoming, we employed CeO2-catalyzed fragmentation of lipids to produce fatty acids using the energy inherent to the MALDI laser as a novel alternative to protein profiling. Fatty acid profiles collected from Enterobacteriaceae, Acinetobacter, and Listeria using CeO2-catalyzed metal oxide laser ionization (MOLI MS), processed by principal component analysis, and validated by leave-one-out cross-validation (CV), showed 100% correct classification at the species level and 98% at the strain level. In comparison, protein profile data from the same bacteria yielded 32%, 54% and 67% mean species-level accuracy using two MALDI-TOF MS platforms, respectively. In addition, several pathogens were misidentified by protein profiling as non-pathogens and vice versa. These results suggest novel CeO2-catalyzed lipid fragmentation readily produced (i) taxonomically tractable fatty acid profiles by MOLI MS, (ii) highly accurate bacterial classification and (iii) consistent strain-level ID for bacteria that were routinely misidentified by protein-based methods.
- Subjects
CERIUM oxides; BACTERIAL typing; FATTY acid analysis; MATRIX-assisted laser desorption-ionization; TIME-of-flight mass spectrometry; METALLIC oxides
- Publication
Scientific Reports, 2015, p10470
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep10470