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- Title
Double Positive CD4 + CD8 + (DP) T-Cells Display Distinct Exhaustion Phenotype in Chronic Hepatitis C.
- Authors
Kochanowicz, Anna Maria; Osuch, Sylwia; Berak, Hanna; Kumorek, Aleksandra; Caraballo Cortés, Kamila
- Abstract
In chronic hepatitis C (CHC), characterized by exhaustion of T-cell function, increased frequencies of double-positive (DP) (CD4+CD8+) cells are present in peripheral blood. We compared the exhaustion phenotype between DP and single positive (SP) T-cells, including HCV-specific cells, and assessed the effect of successful HCV treatment on inhibitory receptors expression. Blood samples from 97 CHC patients were collected before and six months post-treatment. PD-1 (programmed cell death protein 1) and Tim-3 (T-cell immunoglobulin and mucin domain-containing molecule-3) expression was assessed by flow cytometry. DP T-cells displayed significantly higher PD-1 expression, lower Tim-3 expression than CD8+ SP T-cells and lower percentages of PD-1−Tim-3− cells than CD4+ SP T-cells, both before and after treatment. PD-1+Tim-3+ DP T-cells decreased following treatment. HCV-specific cells were more frequent among DP than SP T-cells, both before and after treatment. HCV-specific DP T-cells were characterized by lower PD-1 expression, higher PD-1 and Tim-3 co-expression, and lower percentages of PD-1−Tim-3− cells (both before and after treatment) and higher post-treatment Tim-3 than HCV-specific SP T-cells. Their percentages decreased following treatment, but the exhaustion phenotype remained unchanged. DP T-cells in CHC exhibit a distinct exhaustion phenotype from SP T-cells, and these changes mostly persist following successful treatment.
- Subjects
CHRONIC hepatitis C; CD8 antigen; CD4 antigen; T-cell exhaustion; PHENOTYPES; PROGRAMMED cell death 1 receptors
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 10, p1446
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12101446