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- Title
Improving Schwann Cell Differentiation from Human Adipose Stem Cells with Metabolic Glycoengineering.
- Authors
Du, Jian; Wang, Zihui; Liu, Xiao; Hu, Cecilia; Yarema, Kevin J.; Jia, Xiaofeng
- Abstract
Schwann cells (SCs) are myelinating cells that promote peripheral nerve regeneration. When nerve lesions form, SCs are destroyed, ultimately hindering nerve repair. The difficulty in treating nerve repair is exacerbated due to SC's limited and slow expansion capacity. Therapeutic use of adipose-derived stem cells (ASCs) is emerging in combating peripheral nerve injury due to these cells' SC differentiation capability and can be harvested easily in large numbers. Despite ASC's therapeutic potential, their transdifferentiation period typically takes more than two weeks. In this study, we demonstrate that metabolic glycoengineering (MGE) technology enhances ASC differentiation into SCs. Specifically, the sugar analog Ac5ManNTProp (TProp), which modulates cell surface sialylation, significantly improved ASC differentiation with upregulated SC protein S100β and p75NGFR expression and elevated the neurotrophic factors nerve growth factor beta (NGFβ) and glial cell-line-derived neurotrophic factor (GDNF). TProp treatment remarkably reduced the SC transdifferentiation period from about two weeks to two days in vitro, which has the potential to improve neuronal regeneration and facilitate future use of ASCs in regenerative medicine.
- Subjects
HUMAN stem cells; NERVOUS system regeneration; SCHWANN cells; CELL differentiation; NERVE growth factor; PERIPHERAL nerve injuries
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 8, p1190
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12081190