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- Title
DNA-based genetic markers for rapid cycling Brassica rapa (Fast Plants type) designed for the teaching laboratory.
- Authors
Slankster, Eryn E.; Chase, Jillian M.; Jones, Lauren A.; Wendell, Douglas L.
- Abstract
We have developed DNA-based genetic markers for rapid cycling Brassica rapa RCBr), also known as Fast Plants. Although markers for B. rapa already exist, ours were intentionally designed for use in a teaching laboratory environment. The qualities we selected for were robust amplification in PCR, polymorphism in RCBr strains, and alleles that can be easily resolved in simple agarose slab gels. We have developed two single nucleotidepolymor-phism(SNP) based markers and 14 variable number tandem repeat(VNTR)-type markers spread over four chromosomes. The DNA sequences of these markers represent variation in a wide range of genomic features. Among the VNTR-type markers, there are examples of variation in a non-genic region, variation within an intron, and variation in the coding sequence of agene. Among the SNP-based markers there are examples of polymorphism in intronic DNA and synonymous substitution in a coding sequence. Thus these markers can serve laboratory exercises in both transmission genetics and molecular biology.
- Subjects
DNA; GENETIC markers; CHINESE cabbage; PLANT genetics; BRASSICA
- Publication
Frontiers in Plant Science, 2012, Vol 3, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2012.00118