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- Title
E scherichia coli rad D ( yejH ) gene: a novel function involved in radiation resistance and double-strand break repair.
- Authors
Chen, Stefanie H.; Byrne, Rose T.; Wood, Elizabeth A.; Cox, Michael M.
- Abstract
A transposon insertion screen implicated the yejH gene in the repair of ionizing radiation-induced damage. The yejH gene, which exhibits significant homology to the human transcription-coupled DNA repair gene XPB, is involved in the repair of double-strand DNA breaks. Deletion of yejH significantly sensitized cells to agents that cause double-strand breaks (ionizing radiation, UV radiation, ciprofloxacin). In addition, deletion of both yejH and radA hypersensitized the cells to ionizing radiation, UV and ciprofloxacin damage, indicating that these two genes have complementary repair functions. The Δ yejH Δ radA double deletion also showed a substantial decline in viability following an induced double-strand DNA break, of a magnitude comparable with the defect measured when the recA, recB, recG or priA genes are deleted. The ATPase activity and C-terminal zinc finger motif of yejH play an important role in its repair function, as targeted mutant alleles of yejH did not rescue sensitivity. We propose that yejH be renamed radD, reflecting its role in the DNA repair of radiation damage.
- Subjects
ESCHERICHIA coli; ESCHERICHIA coli DNA; EFFECT of radiation on bacteria; DNA repair; BACTERIAL genes; DNA damage; TRANSPOSONS; IONIZING radiation; BACTERIA
- Publication
Molecular Microbiology, 2015, Vol 95, Issue 5, p754
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.12885