We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of allelic variation in wild potato (Solanum) species by simple sequence repeat (SSR) markers.
- Authors
Tiwari, Jagesh Kumar; Ali, Nilofer; Devi, Sapna; Zinta, Rasna; Kumar, Vinod; Chakrabarti, Swarup Kumar
- Abstract
Allelic variation in wild potato (Solanum) species was analysed using 14 simple sequence repeat (SSR) markers. SSR allelic profiles showed high polymorphism and distinctness among the wild species. A total of 109 alleles of 14 polymorphic SSR markers were scored in 82 accessions belonging to 22 wild potato species. Allele size ranged from a minimum of 104 bp (STI0030) to a maximum of 304 bp (STM5114). Number of SSR alleles per marker ranged from 4 (STM5127/STM1053) to 13 (STM0019), whereas PIC value varied between 0.66 (STM1053) and 0.91 (STM0019). Cluster analysis using SSR allelic profiles of 82 accessions grouped showed 5 major clusters (I–V) based on the Dice similarity coefficient using neighbour-joining clustering method. Distinct allelic variations were observed among the accessions irrespective of the origin country, series and species. Our study suggests that SSR-based molecular characterization of wild potato species is accession specific and development of an allelic dataset for all the accessions would strengthen their utilization in potato research in future.
- Subjects
SOLANUM; POTATOES; MICROSATELLITE repeats in plants; PLANT species; ALLELES; SEQUENCE analysis
- Publication
3 Biotech, 2019, Vol 9, Issue 7, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-019-1785-6