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- Title
ENHANCED RESISTANCE TO Aspergillus flavus IN TRANSGENIC PEANUT HAVING RICE CHITINASE RCG3 GENE.
- Authors
Um-e-Robab; Mahmood, Tariq; Zafar, Yusuf
- Abstract
The herbicide resistance (bar) and rice chitinase (rcg3) genes were incorporated in the commonly cultivated varieties of peanut (BARD-479 and Golden) through Agrobacterium mediated transformation. The role of genetically transformed peanut in controlling aflatoxin contamination by Aspergillus flavus was investigated by HPLC and TLC methods. In case of detection by HPLC the herbicide resistance bar gene had no affect on the aflatoxin content and the aflatoxin B1 found in non transgenic seeds was also present in the transgenic seeds. Transgenic seeds having rcg3 gene controlled the contamination of aflatoxin B1 that appeared in the non transgenic counter parts of the same variety. Whereas in case of aflatoxin determined by TLC method the plants having rcg3 gene also showed the control of Aspergillus flavus in mature peanut seeds.
- Subjects
ASPERGILLUS flavus; CHITINASE; HERBICIDE resistance; PEANUT varieties; AGROBACTERIUM; AFLATOXINS; HIGH performance liquid chromatography; TRANSGENIC seeds
- Publication
Pakistan Journal of Agricultural Sciences, 2014, Vol 51, Issue 2, p283
- ISSN
0552-9034
- Publication type
Article