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- Title
New Aspect of Composition and Biological Properties of Glechoma hederacea L. Herb: Detailed Phytochemical Analysis and Evaluation of Antioxidant, Anticoagulant Activity and Toxicity in Selected Human Cells and Plasma In Vitro.
- Authors
Sławińska, Natalia; Kluska, Magdalena; Moniuszko-Szajwaj, Barbara; Stochmal, Anna; Woźniak, Katarzyna; Olas, Beata
- Abstract
It is known that phenolic compounds can alleviate the negative impact of oxidative stress and modulate hemostasis. However, the effect of extracts and phenolics from Glechoma hederacea L. on the biomarkers of these processes is not well documented. The aim of our study was to investigate the in vitro protective effects of one extract and three fractions (20, 60, and 85% fraction) from G. hederacea L. on oxidative stress and hemostasis. Phytochemical analysis showed that aerial parts of G. hederacea L. are rich in both phenolic acids (such as rosmarinic acid, neochlorogenic acid, and chlorogenic acid) and flavonoids (mainly rutin and glycoside derivatives of apigenin, quercetin, and luteolin). We observed that the 85% fraction (at three concentrations: 5, 10, and 50 μg/mL) inhibited protein carbonylation. Moreover, the extract and 85% fraction (at the concentration of 50 μg/mL) could reduce lipid peroxidation. All fractions and the extract were very effective at decreasing H2O2-induced DNA damage in PBM cells. The 85% fraction had the strongest protective potential against DNA oxidative damage. We also observed that the extract and fractions decreased PBM cell viability to a maximum of 65% after 24 h incubation. Our results indicate that the 85% fraction showed the strongest antioxidant potential. The main component of the 85% fraction was apigenin (26.17 ± 1.44 mg/g), which is most likely responsible for its strong antioxidant properties.
- Subjects
ANTIOXIDANT analysis; IN vitro studies; PHENOLS; FLAVONOIDS; DNA; BLOOD plasma; ANTICOAGULANTS; HEMOSTASIS; PHYTOCHEMICALS; OXIDATIVE stress; CELL survival; FLAVONES; PLANT extracts; DRUG toxicity; LIPID peroxidation (Biology); HYDROGEN peroxide
- Publication
Nutrients, 2023, Vol 15, Issue 7, p1671
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15071671