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- Title
Effect of Lactobacillus rhamnosus hsryfm 1301 Fermented Milk on Lipid Metabolism Disorders in High-Fat-Diet Rats.
- Authors
Qu, Hengxian; Zong, Lina; Sang, Jian; Wa, Yunchao; Chen, Dawei; Huang, Yujun; Chen, Xia; Gu, Ruixia
- Abstract
To further explore and improve the mechanism of probiotics to alleviate the disorder of lipid metabolism, transcriptomic and metabolomic with bioinformatic analysis were combined. In the present study, we successfully established a rat model of lipid metabolism disorder using a high-fat diet. Intervention with Lactobacillus rhamnosus hsryfm 1301 fermented milk resulted in a significant reduction in body weight, serum free fatty acid and blood lipid levels (p < 0.05), which predicted that the lipid metabolism disorder was alleviated in rats. Metabolomics and transcriptomics identified a total of 33 significantly different metabolites and 183 significantly different genes screened in the intervention group compared to the model group. Comparative analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotations identified a total of 61 pathways in which differential metabolites and genes were jointly involved, with linoleic acid metabolism, glycine, serine and threonine metabolism and glutamatergic synapse in both transcriptome and metabolome being found to be significantly altered (p < 0.05). Lactobacillus rhamnosus hsryfm 1301 fermented milk was able to directly regulate lipid metabolism disorders by regulating the metabolic pathways of linoleic acid metabolism, glycerophospholipid metabolism, fatty acid biosynthesis, alpha-linolenic acid metabolism, fatty acid degradation, glycerolipid metabolism and arachidonic acid metabolism. In addition, we found that Lactobacillus rhamnosus hsryfm 1301 fermented milk indirectly regulates lipid metabolism through regulating amino acid metabolism, the nervous system, the endocrine system and other pathways. Lactobacillus rhamnosus hsryfm 1301 fermented milk could alleviate the disorders of lipid metabolism caused by high-fat diet through multi-target synergy.
- Subjects
LIPID metabolism; SERINE metabolism; GLYCINE metabolism; BIOLOGICAL models; BODY weight; CULTURED milk; METABOLOMICS; HUMAN genome; THREONINE; ANIMAL experimentation; GENETIC testing; CELL receptors; PROBIOTICS; METABOLIC disorders; BIOINFORMATICS; COMPARATIVE studies; CELLULAR signal transduction; RATS; GENE expression profiling; LINOLEIC acid; DESCRIPTIVE statistics; LACTOBACILLUS; PHOSPHOLIPIDS; ARACHIDONIC acid; DATA analysis software; DIETARY fats; FATTY acids; LIPIDS
- Publication
Nutrients, 2022, Vol 14, Issue 22, p4850
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14224850