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- Title
Protective effect of betaine against burn-induced pulmonary injury in rats.
- Authors
Şener, Göksel; Şehirli, Ahmet Özer; Satılmış, Burcu; Tetik, Şermin; Çetinel, Şule; Yeğen, Berrak; Aykaç, Aslı
- Abstract
BACKGROUND: This study was designed to determine possible protective effect of betaine treatment against oxidative injury in pulmonary tissue induced with thermal trauma. METHODS: Under ether anesthesia, shaved dorsum of Wistar albino rats was exposed to a 90°C water bath for 10 seconds to induce burn injury. Betaine was administered orally (250 mg/kg) for a period of 21 days before burn injury, and single dose of betaine was administered after thermal injury. Control group rats were exposed to 25°C water bath for 10 seconds. Upon conclusion of experiment, rats were decapitated and blood was collected for analysis of pro-inflammatory cytokines and lactate dehydrogenase (LDH) activity. Lung tissue samples were taken to determine malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO), and Na+K+-ATPase activity, in addition to histological analysis. RESULTS: Burn injury caused significant increase in both cytokine levels and LDH activity. In lung samples, raised MDA levels, MPO activity, and reduced GSH levels and Na+/K+-ATPase activity were found due to burn injury. CONCLUSION: Treatment of rats with betaine significantly restored GSH level and Na+K+-ATPase activity, and decreased MDA level and MPO activity. According to the findings of the present study, betaine significantly diminishes burn-induced damage in tissue.
- Subjects
ADENOSINE triphosphatase; ANALYSIS of variance; ANIMAL experimentation; BATHS; BURNS &; scalds; COMPARATIVE studies; CYTOKINES; EXPERIMENTAL design; LACTATE dehydrogenase; LUNG injuries; PENETRATING wounds; RATS; RESEARCH funding; STATISTICS; TUMOR necrosis factors; MALONDIALDEHYDE; DATA analysis; OXIDATIVE stress; BETAINE; DATA analysis software; DESCRIPTIVE statistics; THERAPEUTICS
- Publication
Turkish Journal of Trauma & Emergency Surgery / Ulusal Travma ve Acil Cerrahi Dergisi, 2016, Vol 22, Issue 5, p417
- ISSN
1306-696X
- Publication type
Article
- DOI
10.5505/tjtes.2015.60137