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- Title
Cell counting chamber vs. Sysmex XN-1000 for determining white blood cell count and differentiation for body fluids.
- Authors
Evren, Ebru; Us, Ebru; Hekimoğlu, Can Hüseyin; Ceren Karahan, Zeynep
- Abstract
Sterile body fluids (BFs) include key information for the diagnosis and monitoring of a variety of diseases. A cornerstone test is the total white blood cell (WBC) count, which comprises the differential of WBC of body fluid analysis. It is important to test automated hematology analyzers that should be verified using patient samples. The aim of this study is to compare both the performance of the Sysmex XN-1000 system's body fluid module for cell counting and differentiation to the results of a cell counting chamber. This study was performed on 200 routinely laboratory sent BFs. Cell counts and differentiation were determined with both bright-lined Neubauer Cell Counting Chamber and Sysmex XN-1000 system body fluid mode. The correlation coefficients of WBC count by two methods indicated very strong correlation (r≥0.90, p<0.0001) for any specimen except pleural fluid. According to Passing-Bablok regression analysis, Sysmex XN-1000 showed acceptable performance according to bias of the slope criteria (<±20%). The XN-1000 hematological analyzer's body fluid mode can rapidly count and identify cells, and it can be used as a simple and rapid screening method for laboratory testing of sterile body fluids, particularly in laboratories with a massive quantity of biological fluids.
- Subjects
LEUKOCYTE count; BODY fluids; CELL differentiation; BODY fluid analysis; REGULATION of body fluids; LEUCOCYTES
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2023, Vol 48, Issue 1, p19
- ISSN
0250-4685
- Publication type
Article
- DOI
10.1515/tjb-2022-0179