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- Title
Bioassay to detect Ascophyllum nodosum extract-induced cytokinin-like activity in Arabidopsis thaliana.
- Authors
Khan, Wajahatullah; Hiltz, David; Critchley, Alan T.; Prithiviraj, Balakrishnan
- Abstract
ophyllum nodosum, a brown macroalga, is the most widely used seaweed in agriculture. We report a rapid method for the detection of cytokinin-like activity in plants treated with a commercial A. nodosum liquid concentrate (Stimplex®) using a transgenic line of Arabidopsis carrying the ARR5 promoter fused to ß-glucuronidase (GUS) reporter gene. Based on GUS activity assay, an increase in cytokinin-like activity was detected in plants grown in vitro treated with 3 mL L Stimplex®, whereas foliar spray treatments showed similar cytokinin-like activity at a concentration of 5 mL L. Histochemical staining showed Stimplex®-induced GUS activity in leaf as well as in the root tissues. Taken together, our results suggest that Stimplex® contains compounds that may elicit endogenous cytokinin-like activity. Furthermore, it is shown that this bioassay can be used for rapid screening of extracts that can stimulate cytokinin-like activities using Arabidopsis AAR5::GUS reporter transgenic plants.
- Subjects
BIOLOGICAL assay; CYTOKININS; ARABIDOPSIS thaliana; PLANT regulators; REPORTER genes; TRANSGENIC plants
- Publication
Journal of Applied Phycology, 2011, Vol 23, Issue 3, p409
- ISSN
0921-8971
- Publication type
Article
- DOI
10.1007/s10811-010-9583-x