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- Title
COUP-TFII Stimulates Dentin Sialophosphoprotein Expression and Mineralization in Odontoblasts.
- Authors
Hur, S.-W.; Oh, S.-H.; Jeong, B.-C.; Choi, H.; Kim, J.-W.; Lee, K.-N.; Hwang, Y.-C.; Ryu, J.-H.; Kim, S.-H.; Koh, J.-T.
- Abstract
Chicken ovalbumin upstream promoter transcription factor 2 (COUP-TFII), an orphan nuclear receptor belonging to the steroid-thyroid hormone receptor superfamily, plays an important role in cell fate determination of various tissues. However, the specific role of COUP-TFII in tooth development has not yet been elucidated. In the present study, we aimed to explore the role of COUP-TFII in dentin sialophosphoprotein (DSPP) expression and matrix mineralization in odontoblast-lineage cells. In primary human dental pulp cells (HDPCs) and murine dental papilla-derived cells (MDPC-23) cultured in a mineralizing medium, the expression of COUP-TFII was induced along with the increased odontoblast-specific dentin matrix protein-1 (DMP-1) and DSPP expression. Endogenous expression of COUP-TFII in maxillary second molar germs of rats showed an increasing tendency as development of the tooth progressed. Also, COUP-TFII protein was detected in greater quantity in the odontoblastic layer of second molar germs than in that of third molar germs of rats. Overexpression of COUP-TFII using an adenoviral system upregulated the expression of odontoblast-specific genes with increased alkaline phosphatase activity and matrix mineralization in odontoblast-lineage cells. In contrast, knockdown of COUP-TFII using small interfering RNA decreased the expression of odontoblast-specific genes, which reduced matrix mineralization. Mechanistic studies revealed that COUP-TFII increased DSPP transcription by direct binding on the DSPP promoter. In addition, COUP-TFII physically interacted with the homeodomain transcription factor Msx2 and antagonistically regulated the Msx2 effect on DSPP promoter activity. Taken together, these results suggest that COUP-TFII has a stimulatory role in DSPP expression and matrix mineralization in odontoblast-lineage cells.
- Subjects
PROTEIN expression; DENTIN; BIOMINERALIZATION; ODONTOBLASTS; OVALBUMINS; TRANSCRIPTION factors; NUCLEAR receptors (Biochemistry); LABORATORY rats
- Publication
Journal of Dental Research, 2015, Vol 94, Issue 8, p1135
- ISSN
0022-0345
- Publication type
Article
- DOI
10.1177/0022034515585125