We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Distinct bactericidal activities of bovine lactoferrin peptides LFampin 268–284 and LFampin 265–284: Asp-Leu-Ile makes a difference.
- Authors
van der Kraan, Marieke I. A.; Nazmi, Kamran; van ’t Hof, Wim; Amerongen, Arie V. Nieuw; Veerman, Enno C. I.; Bolscher, Jan G. M.
- Abstract
Two lactoferrampin (LFampin) peptides derived from bovine lactoferrin were compared with respect to their bactericidal activities. LFampin 265–284 killed a set of Gram-positive bacteria that were resistant to LFampin 268–284. The presence of 265Asp-Leu-267Ile did not simply lead to an overall increased potency, since higher concentrations of LFampin 265–284 than LFampin 268–284 were needed to kill the Gram-negative bacteria that were tested. The Asp-Leu-Ile sequence enhances the propensity of LFampin to adopt an α-helix, as shown by circular dichroism spectroscopy. These results suggest that the helical conformation of the peptide is an important determinant of the susceptibility of Gram-positive bacteria.
- Subjects
LACTOFERRIN; GRAM-positive bacteria; GRAM-negative bacteria; PEPTIDE antibiotics; IRON proteins
- Publication
Biochemistry & Cell Biology, 2006, Vol 84, Issue 3, p358
- ISSN
0829-8211
- Publication type
Article
- DOI
10.1139/o06-042