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- Title
Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.
- Authors
Ouattara, Mahamoudou; Tamboura, Mamadou; Kambiré, Dinanibè; Lê, Kim Anh; Phan, Thanh Van; Velusamy, Srinivasan; Nguyen, Hien Anh; Trang, Dai Vo Thi; Lessa, Fernanda C; Iijima, Makiko; Nguyen, Dac Trung; Schwartz, Stephanie B; McGee, Lesley; Traoré, Rasmata Ouédraogo; Beall, Bernard; Van Phan, Thanh
- Abstract
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
- Subjects
STREPTOCOCCUS pneumoniae; POLYMERASE chain reaction; SEROTYPES; CEREBROSPINAL fluid; STREPTOCOCCAL disease diagnosis; SEROTYPING; RESEARCH; RESEARCH methodology; EVALUATION research; STREPTOCOCCUS; COMPARATIVE studies
- Publication
Journal of Infectious Diseases, 2021, Vol 224, pS204
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiab056