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- Title
Nestin-lineage cells contribute to the microvasculature but not endocrine cells of the islet.
- Authors
Treutelaar, Mary Kay; Skidmore, Jennifer M.; Dias-Leme, Claudia L.; Hara, Manami; Zhang, Lizhi; Simeone, Diane; Martin, Donna M.; Burant, Charles F.
- Abstract
To clarify the lineage relationship between cells that express the neural stem cell marker nestin and endocrine cells of the pancreas, we analyzed offspring of a cross between mice carrying a nestin promoter/enhancer-driven cre-recombinase (Nestin-cre) and C57BL/ 6J-Gtrosa26[sup tm1Sor] mice that carry a loxP-disrupted β-galactosidase gene (Rosa26). In nestin-cre[sup +/tg];R26R[sup loxP/+] embryos, cre-recombinase was detected in association with nestin-positive cells in the pancreatic mesenchyme with some of the nestin-positive cells lining vascular channels. In postnatal mice, pancreatic β-galactosidase expression was restricted to vascular endothelial cells of the islet and a subset of cells in the muscularis of arteries in a distribution identical to endogenous nestin expression. Ex vivo explants of mouse pancreatic ducts grew dense cultures that costained for nestin and β-galactosidase, demonstrating recombination in vitro. The cultures could be differentiated into complex stereotypic structures that contain nestin- and insulin-expressing cells. Nestin-cre[sup +/tg];R26R[sup loxP/+]-derived duct cultures showed that insulin-positive cells were negative for β-galactosidase. These results indicate that both in vivo and in vitro pancreatic endocrine cells arise independently of nestin-positive precursors. The apparent vascular nature of the nestin-positive cell population and the close association with endocrine cells suggest that nestin-positive cells play an important role in the growth and maintenance of the islet.
- Subjects
STEM cells; ISLANDS of Langerhans; INSULIN; ENZYME metabolism; CELL metabolism; PROTEIN metabolism; PROTEINS; IN vitro studies; ANIMAL populations; RESEARCH; BLOOD vessels; NERVE tissue proteins; SMOOTH muscle; EMBRYOS; ENDOTHELIUM; ANIMAL experimentation; PANCREATIC duct; ARTERIES; RESEARCH methodology; CYTOSKELETAL proteins; RNA; MICROCIRCULATION; EVALUATION research; ENDOCRINE cells; COMPARATIVE studies; GLYCOSIDASES; RESEARCH funding; CELL lines; MICE
- Publication
Diabetes, 2003, Vol 52, Issue 10, p2503
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diabetes.52.10.2503