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- Title
Delayed transcapillary transport of insulin to muscle interstitial fluid in obese subjects.
- Authors
Sjöstrand, Mikaela; Sjostrand, Mikaela; Gudbjornsdottir, Soffia; Gudbjörnsdottir, Soffia; Holmang, Agneta; Holmäng, Agneta; Lonn, Lars; Lönn, Lars; Strindberg, Lena; Lonnroth, Peter; Lönnroth, Peter; Sjöstrand, Mikaela; Gudbjörnsdottir, Soffia; Holmäng, Agneta; Lönn, Lars; Lönnroth, Peter
- Abstract
Insulin-resistant subjects have a slow onset of insulin action, and the underlying mechanism has not been determined. To evaluate whether a delayed transcapillary transport is part of the peripheral insulin resistance, we followed the kinetics of infused insulin and inulin in plasma and muscle interstitial fluid in obese insulin-resistant patients and control subjects. A total of 10 lean and 10 obese men (BMI 24 +/- 0.8 vs. 32 +/- 0.8 kg/m(2), P < 0.001) was evaluated during a hyperinsulinemic-euglycemic clamp (insulin infusion rate 120 mU. m(-2). min(-1)) combined with an inulin infusion. Measurements of insulin and inulin in plasma were taken by means of arterial-venous catheterization of the forearm and microdialysis in brachioradialis muscle combined with forearm blood flow measurements with vein occlusion pletysmography. The obese subjects had a significantly lower steady-state glucose infusion rate and, moreover, demonstrated a delayed appearance of insulin (time to achieve half-maximal concentration [T(1/2)] 72 +/- 6 vs. 46 +/- 6 min in control subjects, P < 0.05) as well as inulin (T(1/2) 83 +/- 3 vs. 53 +/- 7 min, P < 0.01) in the interstitial fluid. Also, the obese subjects had a delayed onset of insulin action (T(1/2) 70 +/- 9 vs. 45 +/- 5 min in control subjects, P < 0.05), and their forearm blood flow rate was significantly lower. These results demonstrate a delayed transcapillary transport of insulin and inulin from plasma to the muscle interstitial fluid and a delayed onset of insulin action in insulin-resistant obese subjects.
- Subjects
INSULIN resistance; PEOPLE with diabetes
- Publication
Diabetes, 2002, Vol 51, Issue 9, p2742
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diabetes.51.9.2742