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- Title
Increased hypothalamic neuropeptide Y concentrations in diabetic rat.
- Authors
Williams, Gareth; Steel, Jennifer H.; Cardoso, Helena; Ghatei, Mohammad A.; Lee, Ying C.; Gill, Jaswinder S.; Burrin, Jacky M.; Polak, Julia M.; Bloom, Stephen R.; Williams, G; Steel, J H; Cardoso, H; Ghatei, M A; Lee, Y C; Gill, J S; Burrin, J M; Polak, J M; Bloom, S R
- Abstract
Central and lateral hypothalamic concentrations of 10 regulatory peptides were measured by radioimmunoassay in streptozocin-induced diabetic (STZ-D) and matched control rats between 1 day and 14 wk after diabetes induction. After 2 wk, both central and lateral hypothalamic neuropeptide Y (NPY) concentrations in STZ-D rats were consistently higher than those found in control rats, with significant 30-50% increases at 4 wk in the central hypothalamus, and at 6 and 14 wk in both central and lateral hypothalamus. Immunocytochemical studies in 4- and 6-wk STZ-D animals showed the appearance of intensely NPY-positive swollen cell bodies in the supraoptic nucleus and a subjective increase in NPY staining of medial hypothalamic nerve fibers. Central hypothalamic concentrations of three other peptides were significantly greater in STZ-D animals than those in control animals at single points (neurotensin, 1 day; calcitonin gene-related peptide, 2 wk; neurokinin, 4 wk). Hypothalamic concentrations of the other six peptides examined (bombesin, galanin, neuromedin B, substance P, somatostatin, and vasoactive intestinal peptide) did not differ significantly between STZ-D and control groups at any time. However, galanin immunostaining in the supraoptic and magnocellular paraventricular nuclei was strikingly concentrated in a reduced number of distended cell bodies. Hypothalamic peptide changes in STZ-D could be related to metabolic disturbance, changes in energy and water balance, altered pituitary function, or other factors. Persistently elevated concentrations of NPY, a very potent central stimulant of eating and drinking, may mediate the hyperphagia and polydipsia characteristic of STZ-D.
- Publication
Diabetes, 1988, Vol 37, Issue 6, p763
- ISSN
0012-1797
- Publication type
journal article
- DOI
10.2337/diab.37.6.763