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- Title
The non-regular orbit: three satellite DNAs in Drosophila martensis ( buzzatii complex, repleta group) followed three different evolutionary pathways.
- Authors
Kuhn, Gustavo C. S.; Schwarzacher, Trude; Heslop-Harrison, John S.
- Abstract
The genome of species from the buzzatii cluster ( buzzatii complex, repleta group) is hosted by a number of satellite DNAs (satDNAs) showing contrasting structural characteristics, genomic organization and evolution, such as pBuM- alpha (~190 bp repeats), pBuM- alpha/beta (~370 bp repeats) and the DBC-150 (~150 bp repeats). In the present study, we aimed to investigate the evolution of these three satDNAs by looking for homologous sequences in the genome of the closest outgroup species: Drosophila martensis ( buzzatii complex). After PCR, we isolated and sequenced 9 alpha, 8 alpha/beta and 11 DBC-150 sequences from this species. The results were compared to all pBuM and DBC-150 sequences available in literature. After D. martensis split from the buzzatii cluster some 6 Mya, the three satDNAs evolved differently in the genome of D. martensis by: (1) maintenance of a collection of major types of ancestral repeats in the genome ( alpha); (2) fixation for a single major type of ancestral repeats ( alpha/beta) or (3) fixation for new divergent species-specific repeat types (DBC-150). Curiously, D. seriema and D. martensis, although belonging to different and allopatric clusters, became independently fixed for the same major type of alpha/beta ancestral repeats, illustrating a rare case of parallelism in satDNA evolution. The contrasting pictures illustrate the diversity of evolutionary pathways a satDNA can follow, defining a “non-regular orbit” with outcomes difficult to predict.
- Subjects
DNA; SATELLITE DNA; PHYLOGENY; DROSOPHILA; GENOMES; GENETICS
- Publication
Molecular Genetics & Genomics, 2010, Vol 284, Issue 4, p251
- ISSN
1617-4615
- Publication type
Article
- DOI
10.1007/s00438-010-0564-1