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- Title
An evaluation of photobiomodulation effects on human gingival fibroblast cells under hyperglycemic condition: an in vitro study.
- Authors
Iranpour, Babak; Mohammadi, Kimia; Hodjat, Mahshid; Hakimiha, Neda; Sayar, Ferena; Kharazi Fard, Mohammad Javad; Sadatmansouri, Saeed; Hanna, Reem
- Abstract
An in vitro study was designed to evaluate the effects of photobiomodulation (PBM) with 915-nm diode laser on human gingival fibroblast (HGF) cells under hyperglycemic condition. The HGF cells were cultured in Dulbecco's modified eagle medium (DMEM) medium containing 30 mM glucose concentration for 48 h to mimic the hyperglycemic condition. Subsequently, the cells received three sessions of PBM (915 nm, continuous emission mode, 200 mW, energy density values of 3.2, 6, and 9.2 J/cm2). Twenty-four hours post-irradiation, cell proliferation, expression of interleukin 6 (IL-6), and vascular endothelial growth factor (VEGF) were assessed with MTT and real-time polymerase chain reaction (PCR) tests, respectively. Also, reactive oxygen species (ROS) production was measured using CM-H2DCFDA fluorimetry. No changes were detected in the cell proliferation rate between the high glucose control group and laser-treated cells, while VEGF and IL-6 gene expression levels increased significantly after PBM in the high glucose-treated cells group. ROS level was significantly decreased in the irradiated cells in high-glucose medium compared with the high glucose control group. Our study revealed the inductive role of 915-nm–mediated PBM on VEGF and the inflammatory response while concurrently reducing reactive oxygen species production in HGF cells in hyperglycemic conditions.
- Subjects
PHOTOBIOMODULATION therapy; REACTIVE oxygen species; FIBROBLASTS; GINGIVA; IN vitro studies
- Publication
Lasers in Medical Science, 2023, p1
- ISSN
0268-8921
- Publication type
Article
- DOI
10.1007/s10103-023-03954-4