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- Title
Active Intermediates in Copper Nitrite Reductase Reactions Probed by a Cryotrapping‐Electron Paramagnetic Resonance Approach.
- Authors
Hedison, Tobias M.; Shanmugam, Muralidharan; Heyes, Derren J.; Edge, Ruth; Scrutton, Nigel S.
- Abstract
Redox active metalloenzymes catalyse a range of biochemical processes essential for life. However, due to their complex reaction mechanisms, and often, their poor optical signals, detailed mechanistic understandings of them are limited. Here, we develop a cryoreduction approach coupled to electron paramagnetic resonance measurements to study electron transfer between the copper centers in the copper nitrite reductase (CuNiR) family of enzymes. Unlike alternative methods used to study electron transfer reactions, the cryoreduction approach presented here allows observation of the redox state of both metal centers, a direct read‐out of electron transfer, determines the presence of the substrate/product in the active site and shows the importance of protein motion in inter‐copper electron transfer catalyzed by CuNiRs. Cryoreduction‐EPR is broadly applicable for the study of electron transfer in other redox enzymes and paves the way to explore transient states in multiple redox‐center containing proteins (homo and hetero metal ions).
- Subjects
NITRITE reductase; ELECTRON paramagnetic resonance spectroscopy; PARAMAGNETIC resonance; OXIDATION-reduction reaction; ELECTRON paramagnetic resonance; CHARGE exchange; NITRATE reductase
- Publication
Angewandte Chemie, 2020, Vol 132, Issue 33, p14040
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.202005052