We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Rapid Determination of Major Compounds in the Ethanol Extract of Geopropolis from Malaysian Stingless Bees, Heterotrigona itama, by UHPLC-Q-TOF/MS and NMR.
- Authors
Lingling Zhao; Mengjiao Yu; Minghui Sun; Xiaofeng Xue; Tongtong Wang; Wei Cao; Liping Sun
- Abstract
A reliable, rapid analytical method was established for the characterization of constituents of the ethanol extract of geopropolis (EEGP) produced by Malaysian stingless bees--Heterotrigona itama--by combining ultra-high-performance liquid chromatography with quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). Based on known standards, the online METLIN database, and published literature, 28 compounds were confirmed. Phenolic acids, flavones, triterpenes and phytosterol were identified or tentatively identified using characteristic diagnostic fragment ions. The results indicated that terpenoids were the main components of EEGP, accompanied by low levels of phenolic acids, flavonoids, and phytosterol. Two major components were further purified by preparative high-performance liquid chromatography (PHPLC) and identified by nuclear magnetic resonance (NMR) as 24(E)-cycloart-24-ene-26-ol-3-one and 20-hydroxy-24-dammaren-3-one. These two triterpenes, confirmed in this geopropolis for the first time, are potential chemical markers for the identification of geopropolis from Malaysian stingless bees, H. itama.
- Subjects
ETHANOL; STINGLESS bees; HIGH performance liquid chromatography; PHENOLIC acids; ORGANIC acids
- Publication
Molecules, 2017, Vol 22, Issue 11, p1935
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules22111935