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- Title
Cytotoxicity and Pro-Apoptotic Activity of 2,2'-Bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], a Phenolic Cyclopentenedione Isolated from the Cyanobacterium Strain Nostoc sp. str. Lukešová 27/97.
- Authors
Vacek, Jan; Hrbáč, Jan; Kopecký, Jiří; Vostálová, Jitka
- Abstract
The cytotoxicity of the polyphenol 2,2'-bis[4,5-bis(4-hydroxybenzyl)-2-(4-hydroxyphenyl)cyclopent-4-en-1,3-dione], nostotrebin 6 (NOS-6), was tested under in vitro conditions using mouse fibroblasts (BALB/c cells). Identification of NOS-6 and its uptake into fibroblasts was examined by multi-stage mass spectrometry analysis with the following fragmentation pattern: MS (m/z) [M+H]+ 799.1 → MS2 399.1 → MS3 305.1 → MS4 277.1. Using several cell viability assays, the IC50 of NOS-6 after 24 h incubation was found to be 8.48 ± 0.16/12.15 ± 1.96 μM (neutral red/MTT assay) which was higher than that of doxorubicin. It was found that NOS-6 is capable of inducing both types of cell death, apoptosis and necrosis in a dose-dependent manner. The biological activities of the cyclopentenediones and preliminary data on NOS-6 cytotoxicity are discussed.
- Subjects
CELL-mediated cytotoxicity; POLYPHENOLS; FIBROBLASTS; MASS spectrometry; CELL death
- Publication
Molecules, 2011, Vol 16, Issue 5, p4254
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules16054254