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- Title
Comparing the effect of Toll-like receptor agonist adjuvants on the efficiency of a DNA vaccine.
- Authors
Sajadian, Azadeh; Tabarraei, Alijan; Soleimanjahi, Hoorieh; Fotouhi, Fatemeh; Gorji, Ali; Ghaemi, Amir
- Abstract
We have investigated whether poly(I:C) Toll-like receptor 3 (TLR3) and resiquimod Toll-like receptor 7 (TLR7) agonists can serve as vaccine adjuvants and promote the efficiency of therapeutic DNA vaccination against tumors expressing the human papilloma virus 16 (HPV-16) E7 protein. For this purpose, C57BL/6 mice were inoculated with 2 × 10 TC-1 cells, and they were then immunized with HPV-16 E7 DNA vaccine alone or with 50 μg of resiquimod or poly(I:C) individually. We found that poly(I:C) and resiquimod could induce more antigen-specific lymphocyte proliferation and cytolytic activity compared to vaccination with E7 DNA alone. While E7 DNA had no significant inhibitory effect on tumor growth, co-administration of poly(I:C) and resiquimod with E7 DNA induced significant tumor regression. Peripheral and local cytokine assays demonstrated that co-administration of poly(I:C) and resiquimod with E7 DNA induced circulating antigen-specific IFN-γ and nonspecific intratumoral IL-12. TLR3 and TLR7 agonists can be used to enhance the immune response to DNA vaccine immunogens. Taken together, these data indicate that combined vaccination with DNA encoding HPV-16 E7 plus TLR agonists provides a strategy for improving the efficacy of a vaccine as a possible immunotherapeutic strategy for cervical cancer.
- Subjects
TOLL-like receptors; IMMUNOLOGICAL adjuvants; DNA vaccines; PAPILLOMAVIRUSES; IMMUNIZATION; LYMPHOCYTES; VIROLOGY
- Publication
Archives of Virology, 2014, Vol 159, Issue 8, p1951
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-014-2024-4