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- Title
Construction of an infectious cDNA clone of Culex flavivirus, an insect-specific flavivirus from Culex mosquitoes.
- Authors
Isawa, Haruhiko; Kuwata, Ryusei; Tajima, Shigeru; Hoshino, Keita; Sasaki, Toshinori; Takasaki, Tomohiko; Kobayashi, Mutsuo; Sawabe, Kyoko
- Abstract
Culex flavivirus (CxFV) is an insect-specific flavivirus that has recently been detected in various Culex spp. mosquitoes worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of a Tokyo strain, CxFV-NIID21. The full-length CxFV-NIID21 cDNA was cloned into the low-copy-number plasmid pMW119, which was stably amplified in Escherichia coli. Transfection of a mosquito cell line with in vitro-transcribed RNA from the cDNA clone resulted in the production of recombinant progeny virus with growth properties, cytopathogenicity, and virion morphology similar to the parental virus.
- Subjects
CULEX; ANTISENSE DNA; FLAVIVIRUSES; PLASMIDS; GENE amplification; RECOMBINANT viruses; VIRUS morphology; BACTERIA
- Publication
Archives of Virology, 2012, Vol 157, Issue 5, p975
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-012-1240-z