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- Title
Metabolic engineering of Escherichia coli for production of biodiesel from fatty alcohols and acetyl-CoA.
- Authors
Guo, Daoyi; Pan, Hong; Li, Xun
- Abstract
Microbial production of biodiesel from renewable feedstock has attracted intensive attention. Biodiesel is known to be produced from short-chain alcohols and fatty acyl-CoAs through the expression of wax ester synthase/fatty acyl-CoA: diacylglycerol acyltransferase that catalyzes the esterification of short-chain alcohols and fatty acyl-CoAs. Here, we engineered Escherichia coli to produce various fatty alcohol acetate esters, which depend on the expression of Saccharomyces cerevisiae alcohol acetyltransferase ATF1 that catalyzes the esterification of fatty alcohols and acetyl-CoA. The fatty acid biosynthetic pathways generate fatty acyl-ACPs, fatty acyl-CoAs, or fatty acids, which can be converted to fatty alcohols by fatty acyl-CoA reductase, fatty acyl-ACP reductase, or carboxylic acid reductase, respectively. This study showed the biosynthesis of biodiesel from three fatty acid biosynthetic pathway intermediates.
- Subjects
BIODIESEL fuels; BACTERIAL genetic engineering; ACETYLCOENZYME A; FATTY acid synthesis; BACTERIAL genetics; ESCHERICHIA coli; FATTY alcohols; ESTERIFICATION
- Publication
Applied Microbiology & Biotechnology, 2015, Vol 99, Issue 18, p7805
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-015-6809-5