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- Title
Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell.
- Authors
Xu, Jian; Mon, Hiroaki; Kusakabe, Takahiro; Li, Zhiqing; Zhu, Li; Iiyama, Kazuhiro; Masuda, Atsushi; Mitsudome, Takumi; Lee, Jae
- Abstract
The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAi-mediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS.
- Subjects
RNA interference; SILKWORMS; NUCLEOPOLYHEDROVIRUSES; RECOMBINANT baculoviruses; DOUBLE-stranded RNA; PROTEIN expression
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 24, p10435
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-5279-x