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- Title
Genome editing with engineered zinc finger nucleases.
- Authors
Urnov, Fyodor D.; Rebar, Edward J.; Holmes, Michael C.; Zhang, H. Steve; Gregory, Philip D.
- Abstract
Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and induced pluripotent stem cells, targeted integration in crop plants, and HIV resistance in immune cells - this broad range of outcomes has resulted from the application of the same core technology: targeted genome cleavage by engineered, sequence-specific zinc finger nucleases followed by gene modification during subsequent repair. Such 'genome editing' is now established in human cells and a number of model organisms, thus opening the door to a range of new experimental and therapeutic possibilities.
- Subjects
GENOMES; GENETIC engineering of crops; CYTOGENETICS; DNA repair; NATURAL immunity; NUCLEASES; AMINO acids; ANIMALS; ESTERASES; GENETIC techniques
- Publication
Nature Reviews Genetics, 2010, Vol 11, Issue 9, p636
- ISSN
1471-0056
- Publication type
journal article
- DOI
10.1038/nrg2842