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- Title
ISOLATION AND ANALYSIS OF THE MMPS (GELATINASE) IN BAMBOO AND SPINACH BY GELATIN ZYMOGRAPHY.
- Authors
Kamble, Suresh; Bhagiya, Sanjay G.; Shinde, Keshav; Vikhe, Ganesh
- Abstract
The MMPs (Matrix Metallo-Proteases) are zinc-dependent endopeptidases known for their ability to cleave one or several ECM (extra-cellular matrix) constituents. They exhibit the characteristics HEXXH consensus sequences. The two histidine (H) residues serve as zinc ligands and the glutamic acid (E) residue polarizes a water molecule involved in nucleophilic attack at the scissile peptide bond of the substrate. Plant metallo-proteases are less studied than human or snake venom metallo-proteases. They were proposed to play a role in plant development and senescence. It is also found that GmMMP-2 may play a role in a novel defence mechanism in plants against pathogenic infections. In present study, we have analyzed MMPs (Gelatinase) from the leaves of the Bamboo and Spinach by gelatin Zymography. In case of Bamboo, Protein extracted from mature leaves shows one gelatin digested band (Gelatinase), while in immature leaves, it shows two bands indicating that it contains more than one MMPs having gelatine as target substrate. Protein extracted from Spinach leaves also shows Gelatinase activity.
- Subjects
BAMBOO; GELATIN; SPINACH; HISTIDINE; LIGANDS (Biochemistry)
- Publication
Biochemical & Cellular Archives, 2018, Vol 18, Issue 1, p601
- ISSN
0972-5075
- Publication type
Article