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- Title
Intestinal growth and morphology is associated with the increase in heat shock protein 70 expression in weaning piglets through supplementation with glutamine.
- Authors
Zhong, X.; Zhang, X. H.; Li, X. M.; Zhou, Y. M.; Li, W.; Huang, X. X.; Zhang, L. L.; Wang, T.
- Abstract
The objectives of this study were to determine the effects of oral Gln supplementation on growth performance, intestinal morphology, and expression of heat shock protein (Hsp) 70 in weaning piglets. A total of 65 piglets after weaning at 21 d of age (d 0) were used in this experiment. Five piglets were randomly selected and sacrificed initially at d 0 to determine baseline values for the expression of Hsp70 in the small intestine. The remaining piglets were randomly assigned to 1 of 2 treatments and received 0 or 1 g of oral Gln per kg BW every 12 h. After piglets were humanly sacrificed at d 3, 7, and 14 postweaning, the duodenum, jejunum, and ileum of piglets were sampled to evaluate intestinal morphology and expression and localization of Hsp70. The results indicated that oral Gln supplementation increased plasma concentrations of Gln compared with control piglets (P < 0.05). The ADG and ADFI were greater in piglets supplemented Gln orally than in control piglets during the whole period (P < 0.05). The incidence of diarrhea in piglets supplemented Gln orally was 24% lower numerically than that in control piglets on 8 to 14 d after weaning. The jejunum and the ileum weights were greater in piglets supplemented Gln orally than those in control piglets relative to BW on d 14 postweaning (P < 0.05). Compared with control piglets, villus height and villus height/crypt depth ratio in the jejunum and the ileum were higher in piglets receiving oral Gln on d 14 postweaning (P < 0.05). These results indicated that Gln supplementation can influence the morphology of intestine in weaned piglets. The expression of hsp70 mRNA and proteins in the duodenum and jejunum were greater in piglets supplemented Gln than in control piglets (P < 0.05). However, Gln supplementation had no effect on expression of hsp70 mRNA and proteins in the ileum. Meanwhile, the localization of Hsp70 in the cytoplasm indicated that Hsp70 has a cytoprotective role in epithelial cell function and structure. These results indicated that Gln supplementation is beneficial for intestinal health and development and thus mitigate diarrhea and improve growth performance. The protective mechanisms of Gln in intestine may be associated with increase of Hsp70 expression.
- Subjects
HEAT shock proteins; PIGLETS; GLUTAMINE; DIETARY supplements; ANIMAL nutrition; DUODENUM; JEJUNUM; ILEUM; ANIMAL health
- Publication
Journal of Animal Science, 2011, Vol 89, Issue 11, p3634
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2010-3751