We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phenolic characterization, antioxidant and α-glycosidase inhibitory activities of different fractions from Prinsepia utilis Royle seed shell using in vitro and in silico analyses.
- Authors
Zheng, Xiuqing; Pan, Fei; Zhao, Shuai; Zhao, Lei; Yi, Junjie; Cai, Shengbao
- Abstract
In this study, the free, esterified, and insoluble-bound phenolics in Prinsepia utilis Royle seed shell were identified, and their antioxidant and α-glycosidase inhibitory activities were studied. The main α-glycosidase inhibitor was screened by molecular docking and in vitro experiments. The interaction mechanism between the main inhibitor and α-glycosidase was studied by molecular dynamics simulation. A total of 18 phytochemicals including 14 phenolic compounds were identified, of which rutin and (−)-epicatechin were the main phenolic compounds. The esterified phenolic extract had the strongest antioxidant activity, while the free phenolic extract showed the best α-glycosidase inhibitory activity. Rutin was found to be the main inhibitor of α-glycosidase and can effectively bind to the active site of α-glycosidase through hydrogen bonds and van der Waals force. This research demonstrated the potentials of P. utilis seed shell as a good source of phenolic compounds that can further be utilized for food and/or pharmaceutical applications.
- Subjects
PLANT phenols; VAN der Waals forces; MOLECULAR dynamics; GLYCOSIDASES; PHENOLS; MOLECULAR docking; SEEDS
- Publication
European Food Research & Technology, 2023, Vol 249, Issue 2, p375
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-022-04123-7